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Materials Engineering of Microalgae-Derived Protein Ingredients: Processing-Structure-Property-Performance Relationships, Scale-Up, and Economic Considerations
), Rae Secioria(2), Afrilia Fahrina(3), Muhammad Roil Bilad(4),
(1) Universitas Brawijaya
(2) Ghifra Institute
(3) Cendekia Berkarya Mandiri Foundation
(4) Universiti Brunei Darussalam
Corresponding Author
Abstract
Microalgae-derived proteins are promising functional food materials whose performance depends on cell architecture, protein molecular state, particle structure, associated components, and processing history. This review examines microalgal protein ingredients through a processing-structure-property-performance framework covering cell disruption, fractionation, concentration, and drying. Processing conditions influence protein accessibility, conformation, aggregation, particle size, surface characteristics, and interactions with pigments, lipids, and polysaccharides, thereby governing solubility, surface charge, rheology, emulsification, gelation, dispersibility, and stability. Particular attention is given to trade-offs between protein recovery and functional preservation, together with scale-up constraints and economic considerations related to processing severity, energy demand, ingredient yield, and co-product value.
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References
Dejesús Prieto Garcete, M. R., Ersinger, V. F. A., Vittone, M. D. C., Erba, I. E. D., Cativa, N. M., and Lamas, D. L. (2025). Effect on protein content and digestibility of traditional bread by incorporating Arthrospira platensis. Journal of Applied Phycology, 37(5), 3609-3620.
Severo, I. A., De Lira, G. S., Ambati, R. R., Gokare, R. A., Vargas, J. V. C., Ordonez, J., and Mariano, A. B. (2024). Disruptive potential of microalgae proteins: Shaping the future of the food industry. Future Foods, 9, 100318.
Batista de Oliveira, T. T., Miranda dos Reis, I., Bastos de Souza, M., da Silva Bispo, E., Fonseca Maciel, L., Druzian, J. I., Lordelo Guimarães Tavares, P. P., de Oliveira Cerqueira, A., dos Santos Boa Morte, E., Abreu Glória, M. B., Lima Deus, V., and Radomille de Santana, L. R. (2021). Microencapsulation of Spirulina sp. LEB-18 and its incorporation in chocolate milk: Properties and functional potential. LWT, 148, 111674.
Wang, Y., Tibbetts, S., and McGinn, P. (2021). Microalgae as sources of high-quality protein for human food and protein supplements. Foods, 10, 3002.
Rahman, M. M., Hosano, N., and Hosano, H. (2022). Recovering microalgal bioresources: a review of cell disruption methods and extraction technologies. Molecules, 27(9), 2786.
Timira, V., Meki, K., Li, Z., Lin, H., Xu, M., and Pramod, S. N. (2022). A comprehensive review on the application of novel disruption techniques for proteins release from microalgae. Critical Reviews in Food Science and Nutrition, 62(16), 4309-4325.
Xu, Y., Tong, X., Lu, Y., Lu, Y., Wang, X., Han, J., Liu, Z., Ding, J., Diao, C., Mumby, W., Peng, Y., and Sun, Q. (2024). Microalgal proteins: Unveiling sustainable alternatives to address the protein challenge. International Journal of Biological Macromolecules, 276, 133747.
Patel, P., Pang, Y. L. J., Choi, W. J., and Wong, A. (2025). Protein extraction and isolation from legumes and algae: An industry primer. Food and Bioprocess Technology, 18(10), 8380-8408.
Muys, M., Sui, Y., Schwaiger, B., Lesueur, C., Vandenheuvel, D., Vermeir, P., and Vlaeminck, S. E. (2019). High variability in nutritional value and safety of commercially available Chlorella and Spirulina biomass indicates the need for smart production strategies. Bioresource Technology, 275, 247-257.
Brückner, K., and Griehl, C. (2023). Permeabilization of the cell wall of Chlorella sorokiniana by the chitosan-degrading protease papain. Algal Research, 71, 103066.
Weber, S., Grande, P. M., Blank, L. M., and Klose, H. (2022). Insights into cell wall disintegration of Chlorella vulgaris. PLoS ONE, 17(1), e0262500.
Lima, V. S., de Oliveira, D. R. B., da Silva, C. A. S., Santana, R. de C., Soares, N. de F. F., de Oliveira, E. B., Martins, M. A., and Coimbra, J. S. dos R. (2023). Stabilization of oil-water emulsions with protein concentrates from the microalga Tetradesmus obliquus. Journal of Food Science and Technology, 60(2), 797-808.
Hosseinkhani, N., McCauley, J. I., and Ralph, P. J. (2022). Key challenges for the commercial expansion of ingredients from algae into human food products. Algal Research, 64, 102696.
Dibenedetto, A., Colucci, A., and Aresta, M. (2016). The need to implement an efficient biomass fractionation and full utilization based on the concept of “biorefinery” for a viable economic utilization of microalgae. Environmental Science and Pollution Research, 23(22), 22274-22283.
Novoveská, L., Nielsen, S. L., Eroldoğan, O. T., Haznedaroglu, B. Z., Rinkevich, B., Fazi, S., Robbens, J., Vasquez, M., and Einarsson, H. (2023). Overview and challenges of large-scale cultivation of photosynthetic microalgae and cyanobacteria. Marine Drugs, 21(8), 445.
Harutyunyan, B., Mühlstädt, G., Seibel, L., Hilpmann, G., Sayadyan, A., Aghabekyan, A., Zakoyan, A., Khachatryan, G., Hovhannisyan, R., Stepanyan, T., Harutyunyan, S., and Goginyan, V. (2025). Algal biomass as a food and feed supplement: Process engineering analysis. Functional Foods in Health and Disease, 15(7), 380-395.
Taniguchi, M., Kuda, T., Shibayama, J., Sasaki, T., Michihata, T., Takahashi, H., and Kimura, B. (2019). In vitro antioxidant, anti-glycation and immunomodulation activities of fermented blue-green algae Aphanizomenon flos-aquae. Molecular Biology Reports, 46(2), 1775-1786.
Ibrahim, R. A., Ibrahim, O. A., Tawfek, M. A., Ali, S. I., and El-Baz, F. K. (2023). Functional ricotta cheese with Dunaliella salina algae emulsion. Carpathian Journal of Food Science and Technology, 15(4), 69-90.
Beal, C. M., Gerber, L. N., Thongrod, S., Phromkunthong, W., Kiron, V., Granados, J., Archibald, I., Greene, C. H., and Huntley, M. E. (2018). Marine microalgae commercial production improves sustainability of global fisheries and aquaculture. Scientific Reports, 8(1), 15064.
Martins, V. F. R., Lopes, A. I., Gomes, D., Parreira, C., Badenes, S. M., Costa, L., Pintado, M., Morais, A. M. M. B., and Morais, R. M. S. C. (2025). Unravelling the potential of seven microalgae species: Nutritional, antioxidant, and antimicrobial properties and application. Applied Sciences, 15(12), 6691.
Ansari, F., Nasr, M., Guldhe, A., Gupta, S., Rawat, I., and Bux, F. (2020). Techno-economic feasibility of algal aquaculture via fish and biodiesel production pathways: A commercial-scale application. Science of the Total Environment, 704, 135259.
Yaakob, M. A., Mohamed, R. M. S. R., Al-Gheethi, A., Tiey, A., and Kassim, A. H. M. (2019). Optimising of Scenedesmus sp. biomass production in chicken slaughterhouse wastewater using response surface methodology and potential utilisation as fish feeds. Environmental Science and Pollution Research, 26(12), 12089-12108.
Bao, B., Thomas-Hall, S. R., and Schenk, P. M. (2022). Fast-tracking isolation, identification and characterization of new microalgae for nutraceutical and feed applications. Phycology, 2(1), 86-107.
García-Segovia, P., Pagán-Moreno, M. J., Lara, I. F., and Martínez-Monzó, J. (2017). Effect of microalgae incorporation on physicochemical and textural properties in wheat bread formulation. Food Science and Technology International, 23(5), 437-447.
Chen, H., and Wang, Q. (2022). Microalgae-based green bio-manufacturing-How far from us. Frontiers in Microbiology, 13, 832097.
Chen, C., Tang, T., Shi, Q., Zhou, Z., and Fan, J. (2022). The potential and challenge of microalgae as promising future food sources. Trends in Food Science and Technology, 126, 99-112.
Fanari, F., Comaposada, J., Aymerich, T., Claret, A., Guerrero, L., and Castellari, M. (2026). Protein-enriched fruit smoothies with wet spirulina biomass: high-pressure processing (HPP) and quality stability evaluation. Food and Bioprocess Technology, 19(1), 17.
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