Anti-Inflammatory Activity of Kalanchoe Pinnata (B. Pinnatum) Stem Extract on Acetic Acid-Induced Inflammation in Mice (M. Musculus)

Abstract

The aim of this study was to test the anti-inflammatory activity of Kalanchoe Pinnata (Bryophyllum pinnatum) stem through feeding its infusion to mice (Mus musculus) injected intramuscularly with acetic acid to induce inflammations. The Complete Randomized Design (CDR) under the experimental method was employed wherein the five set-ups had been replicated three times: T0 (10 mg/kg aspirin in 10 ml/kg distilled water), T1 (10 ml/ kg distilled water), T2 (10 v/v Kalanchoe pinnata stem extract at 10 ml/kg), T3 (50 v/v Kalanchoe pinnata stem extract at 10 ml/kg), and T4 (80 v/v Kalanchoe pinnata stem extract at 10 ml/kg). Their anti-inflammatory activities were acquired through the mean percentage score. Meanwhile, Analysis of Variance (ANOVA) was used to validate the significant difference between the treatments administered. The findings showed that after 4 hours, the average gain in thickness due to inflammation in the mice’s (Mus musculus) hind legs were minimized by the treatments: T0 = ~0.79 mm; T1 = ~1.51 mm; T2 = ~1.46 mm; T3 = ~1.36 mm; and, T4 = ~1.16 mm. Moreover, the ANOVA showed that the p-value calculated was lower than the significance level under which the data was tested (0.0000<.01). It was concluded that given at high concentrations, Kalanchoe pinnata (Bryophyllum pinnatum) stems are anti-inflammatory. This entailed that the plant part has considerable effectiveness against inflammations.